Brucella canis testing protocol

Ms Buckley made this Freedom of Information request to Animal and Plant Health Agency Automatic anti-spam measures are in place for this older request. Please let us know if a further response is expected or if you are having trouble responding.

The request was refused by Animal and Plant Health Agency.

Dear Animal and Plant Health Agency,

I would like to follow up on the Brucella canis testing request made by Andy Diament and your response that I have just noticed. I see that it was refused by yourselves on the grounds of cost.
I have identified the questions that I believe should be easily answered by yourselves and adapted them where not so I hope there should now be no barrier to them being answered.
SAT test:
1. Is this test validated for use by APHA with a cut off of ≥ 2/50? Is the specificity and
sensitivity reported for the test based on this cut off?
2. If so, how many dogs formed the control group (presumed Brucella canis free dogs)
used to validate this test?
3. Do you run this test singularly, in duplicate, triplicate, or greater, in respect of any
individual dog sample submitted for analysis?
4. If you test a given sample more than once, HOW FREQUENTLY (e.g. after how many samples are run, daily, weekly, etc) do you review SAT results regularly to
assess reliability of the test? Do you keep a written record of these and their outcome? If so, is this in the form of % of intra-assay results that occur?
RSA test:
RSA test:
5. Was this test, as used by APHA, validated?
6. If so, how many dogs were used as the BC free control group?
7. Do run this test singularly, in duplicate, triplicate, or greater, in respect of any
individual dog sample submitted for analysis?
8. If you test a given sample more than once, what is your decision-making algorithm
for reporting the result if the result varies?
9. If you test a given sample more than once, HOW FREQUENTLY (e.g. after how many samples are run, daily, weekly, etc) do you review SAT results regularly to
assess reliability of the test? Do you keep a written record of these and their outcome? If so, is this in the form of % of intra-assay results that occur?
ELISA:
10. Do you run this test singularly, in duplicate, triplicate, or greater, in respect of any
individual dog sample submitted for analysis?
11. If you test a given sample more than once, what is your decision-making algorithm
for reporting the result if the result varies?
12. If you test a given sample more than once, do you review ELISA results regularly
to assess reliability of the test?
13. If you test a given sample more than once, HOW FREQUENTLY (e.g. after how many samples are run, daily, weekly, etc) do you review SAT results regularly to
assess reliability of the test? Do you keep a written record of these and their outcome? If so, is this in the form of % of intra-assay results that occur?

I hope that you will find this version of questions satisfactory. They have been designed to be answerable very quickly and should take limited time on the part of your team as it is appreciated that you are busy.

Yours faithfully,

Ms Buckley

APHA Access to Information Team (ATI),

Our ref: FOI2023/16758

Dear Ms Buckley

Thank you for your email which we received on the 29th August requesting
information. Your request is being considered in respect to the access to
information legislation.

We aim to answer your request no later than 26th September, which is 20
working days from the date we received it. If we are unable to meet this
deadline we will contact you to explain the reason.

If you have any queries about this email, please contact us at the email
address below.

Yours sincerely

Access to Information Team

[1][Animal and Plant Health Agency request email]

References

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Dear Animal and Plant Health Agency,
Many thanks for your helpful response.
I understand that you also undertook Brucella canis testing of human samples during part of this period. Can you confirm please whether any of the sample sizes reported for the individual tests included human samples? If so, which tests? and what is the corrected figure once human samples are removed? As I was asking for dog data it is pertinent to request this clarification as it is not clear from the reporting.
Many thanks.

Yours faithfully,

Ms Buckley

Dear Animal and Plant Health Agency,

Please ignore the message sent on 07 09 2023. This clarification is for a different request and I will resubmit this there. For avoidance of doubt, the original request sent on the 31.08.2023 in this thread stands.

Yours faithfully,

Ms Buckley

APHA Access to Information Team (ATI),

Our ref: FOI2023/17627

Dear Ms Buckley

Thank you for your email which we received on the 7th September requesting
information. Your request is being considered in respect to the access to
information legislation.

We aim to answer your request no later than 5th October, which is 20
working days from the date we received it. If we are unable to meet this
deadline we will contact you to explain the reason.

If you have any queries about this email, please contact us at the email
address below.

Yours sincerely

Access to Information Team

[1][Animal and Plant Health Agency request email]

References

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1. mailto:[Animal and Plant Health Agency request email]

APHA Access to Information Team (ATI),

2 Attachments

Dear Ms Buckley,

Please find attached the response to your request of 29th August for
information on Brucella canis testing.

Yours sincerely

Access to Information Team

[1][Animal and Plant Health Agency request email]

References

Visible links
1. mailto:[Animal and Plant Health Agency request email]

APHA Access to Information Team (ATI),

2 Attachments

Dear Ms Buckley,

Please find attached the response to your request of 7th September for
information on Brucella Canis - Human Sample Testing.

Yours sincerely

Access to Information Team

[1][Animal and Plant Health Agency request email]

References

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1. mailto:[Animal and Plant Health Agency request email]